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DNA Testing and Technologies
This is the most sensitive test for human saliva. The assay uses a dual monoclonal antibody immunochromatographic assay, specific for human salivary amylase.
The test can detect saliva from envelope flaps, stamps, glass bottles, cans, swabs, plastic lids, and other forensic exhibits BEFORE they are processed for STR analysis.
The suggested protocol is designed such that if saliva is detected, there should be sufficient biological material for an STR profile.
Saliva/amylase detection using antibody to amylase:
Quarino L, Dang Q, Hartmann J, Moynihan N.
An ELISA method for the identification of salivary amylase.
J Forensic Sci. 2005 Jul;50(4):873-6.
Aluoch AO, Sadik OA, Bedi G.
Development of an oral biosensor for salivary amylase using a monodispersed silver for signal amplification.
Anal Biochem. 2005 May 1;340(1):136-44.